Use of Spermac Staining Technique in the Determination of Acrosomal Defects in Cat Semen

The types and rates of acrosome and other (head, mid-piece and tail) abnormal spermatozoon types were determined by the Spermac staining technique. Semen from 5 stray tom cats under the same management conditions was used. Semen collection was performed under general anesthesia by electro-ejaculator once a week for 5 weeks. After ejaculation the semen was diluted by 100 μl of 0.9% NaCl solution and stained with Spermac stain for morphological evaluation. The morphological criteria were acrosome, other (head, mid-piece and tail) and total morphological defect rates, and were 9.20 ± 2.55%, 9.20 ± 3.49% and 18.40 ± 4.79%, respectively. It was concluded at the end of the study that the Spermac staining technique could provide a detailed observation of cat spermatozoa, especially the acrosomal region, and could be employed in the determination of acrosomal defects.